A Novel GH10 Xylanase Xynl3-3 from Alkaline Soil: Gene Cloning and Heterogenous Expression

摘要

Xylanase is the most critical xylan degrading glycoside hydrolases, xylan could be degraded into oligosaccharides, which could be further degraded to xylose by glucosidase and other side-chain Hydrolase. In this study, a novel GH family 10 Xyn13-3 from the genomic DNA of Alkaline Soil was cloned and successfully expressed in Pichia pastoris GS115. The deduced proteins showed low identities with known fungal xylanases which originated from Trichoderma gamsii (61%). The specific activity of purified Xyn13-3 measured by DNS method was 135.24 U/mg. This is a new xylanase that needs further investigation to characterize its use. In order to improve the activity of the recombinant xylanase, severals factors for the optimization of induced culture will be studied, including induction timing, induced time, inducer concentration and inducing temperature, as well as the following study on enzymatic properties which can provide worthful theory data for application.