The Effect of Peptide Affinity in Neural Stem Cell Mechanosensing

摘要

Conclusions: NSC neuronal differentiation was confirmed upon withdraw of FGF2. Culture with 0.5mM soluble RGD showed that cells altered their morphology demonstrating that the NSCs use integrins for cell adhesions to the substrate, further motivating our hypothesis. Additionally, NSCs adhered to PEG scaffolds with a range of peptide concentration and affinity. Current work includes examining adhesion on an expanded stiffness range and further analyzing peptide concentration, as this affects peptide spacing. Future work will demonstrate the time dependent differentiation of NSCs over the range of stiffness with both RGD peptides to more clearly investigate the impact of mechanotransduction on differentiation.