Elucidating Endothelial Cell Hemostatic Regulation by Isolating Integrin-mediated Adhesion to Bioactive Hydrogels

摘要

Conclusions: We have demonstrated a method to isolate individual integrin interactions of HUVECs on ECM hydrogels. This will allow us to then correlate changes in hemostatic regulator protein gene expression, specifically TF, TFPI, ADAMTS-13, VWF, tPA, eNOS, and PAI-1, to specific integrin-mediated attachment and elucidate individual and synergistic effects. Integrin-targeting proteins can then be utilized in hydrogel coatings to promote not only EC attachment and migration, but also a thromboresistant phenotype.