Fast detection for newcastle disease virus by using 81° tilted fiber grating immunosensor

摘要

A type of newcastle disease virus (NDV) immunosensor based on 81° tiled fiber grating (81° TFG) was proposed. Staphylococcus aureus Protein A (SPA) was used to modify the surface of 81° TFG, then highly purified NDV monoclonal antibodies (MAbs) were immobilized on 81° TFG surface through SPA molecules. The experimental results show that, the immunosensor has a lowest limit of detection (LOD) of 0.1~0.2ng/mL to NDV and become saturated at ~1.0ng/mL, and has good linearity with R² of ~0.982 and sensitivity of ~342pm/(ng/mL) in the concentration range of 0~1.0ng/mL.