Application of Cell Analysis and Sorting Techniques to Disease Detection (Rapid Differential Diagnosis of Newcastle Disease Virus)

摘要

Several strains of Newcastle Disease Virus (NDV) have been isolated from chickens in the United States;most strains are relatively harmless and result only in loss of production. Recent outbreaks (March 1972 to present) of a new and virulent strain that produces death in less than 6 days have necessitated a rapid differential test for identification of this particular strain. The plaque assay designed to differentiate velogenic strains of NDV requires 7-10 days for completion. We have developed a rapid differential assay that requires about 2-3 days to complete.