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Composition and in vitro antioxidants activity of Chamaerops humilis L.

机译:Chamaerops humilis L.的组成和体外抗氧化活性

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The aim of this work is to evaluate the polyphenols composition and the antioxidant activity of methanol extracts of Chamaerops humilis L. Methanol extracts from dried leaves of Chamaerops humilis were prepared using Soxhlet extraction and examined as potential sources of phenolic compounds. Different methods were used to evaluate antioxidant activity of the extracts, including colored (ABTS*+), DPPH radical scavenging assay and reducing power. Total phenolic and flavonoid contents of the extracts were evaluated by Folin-Ciocalteu and aluminum chloride (AlCl3) methods, respectively. Phenolic compositions of the methanol extracts were elucidated by high performance liquid chromatography coupled on-line with tandem mass spectrometry (HPLC-MS/MS). The extract was mainly composed of C- and O-flavones and its O-methylated derivatives. The results suggest that methanol extracts have good potential as sources of bioactive compounds and presents an important antioxidants capacity, which can ensures its potential recommendation for application in the pharmaceutical and nutraceutical sectors.
机译:这项工作的目的是评估Chamaerops humilis L甲醇提取物的多酚组成和抗氧化活性。使用Soxhlet提取方法从Chamaerops humilis干燥叶中提取甲醇提取物,并将其作为酚类化合物的潜在来源。不同的方法用于评估提取物的抗氧化活性,包括有色(ABTS * +),DPPH自由基清除试验和还原能力。提取物中的总酚和类黄酮含量分别通过Folin-Ciocalteu和氯化铝(AlCl3)方法进行评估。通过高效液相色谱-串联质谱联用(HPLC-MS / MS)阐明了甲醇提取物的酚类成分。提取物主要由C-和O-黄酮及其O-甲基化衍生物组成。结果表明,甲醇提取物具有作为生物活性化合物来源的良好潜力,并具有重要的抗氧化剂能力,这可以确保其在制药和营养保健领域的潜在推荐应用。

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