Malaria is a disease generally found in a tropical area including Thailand. It is widely known that the biologicaltechnique such as PCR normally used for an accurate detection of malaria-infected blood requiring a considerable periodto repeat the process. Raman spectroscopy is considered to be an alternative method for the malaria infected blooddetection. Theoretically, Raman spectroscopy is based on the scattering process that is less likely in a normal situation.Therefore, an enhancing technique known as “surface-enhanced Raman scattering (SERS)” is required for the signalaugmentation. The SERS provides the enhancement of the electric field near the surface of the substrate. With theapplication of the technique, the main target of this research focuses on the comparison of the SER Raman spectra of thenormal red blood cell and malaria infected red blood cell. However, only a single spectrum cannot provide a cleardifference between the normal and the infected blood. An additional tool for even more effective discrimination wasprovided by using PCA analysis. In the sample preparation stage, the spin coating process was applied to spread the redblood cells uniformly on the surface. In addition, the spectra of the red blood cells including media were collected invarious conditions in terms of the excitation wavelengths and the types of substrate. This additional information can beused as references for any red blood cell related investigation using Raman spectroscopy.
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