AGPDMS electrodes for single cell impedance spectroscopy

摘要

This paper presents a microfluidic device with wide channels and embedded AgPDMS electrodes for measuring the electrical properties of single cells. The work demonstrates the feasibility of using a large channel design and embedded electrodes for impedance spectroscopy to circumvent issues such as channel clogging and limited device re-usability. AgPDMS electrodes were formed on channel sidewalls for impedance detection and cell characterization. Equivalent circuit models were used to interpret multi-frequency impedance data to quantify each cell's cytoplasm conductivity and specific membrane capacitance. Comparisons were made by measuring two leukemia cell lines (AML-2 and HL-60) which were found to have different cytoplasm conductivity values (0.29 ± 0.15 S/m vs. 0.47 ± 0.20 S/m) and specific membrane capacitance values (41 ± 25 mF/m vs. 55 ± 26 mF/m).