Recently, greater attention is devoted towards development of modern polymeric biomaterials of particular applications, mainly hydrogels defined also as superabsorbent polymers (SAP). Hydrogels based on chitosan and natural polymers constitute significant source of biocompatible, biodegradable matrices widely applied in biomedicine. The main assets of biomaterials constitute: biocompatibility, lack of allergic, toxic, mutagenic reactions, lack of immune response and inflammations. In this study chitosan based hydrogels were formed by exposure to UV light, applying PEGOA 700 as crosslinking agent and 2-hydroxy-2-methylpropiophenone as photoinitiator. Initial mixture exposed to UV-light was comprised of 3% of chitosan, 2% of gelatin and accordingly: 1ml (1), 3ml (2) and 5ml (3) of silver nanoparticles (260ppm). Another samples 4 and 5 contained the same reagents as sample 2 and 3 but were enriched in 5ml of 0.3% aqueous solution of albumin. The main objective of performed analyses was based on evaluation of cytotoxicity performed on the cells of human dermis in vitro (BJ, ATCCO CRL-2522TM) according to MTT and XTT methods. The attained results are listed in tables 1 and 2. Table 1. Determination of cytotoxicity of hydrogel's extracts on human dermis cells applying MTT method. Table 2. Determination of cytotoxicity of hydrogel's extracts on human dermis cells applying XTT method. Received hydrogel matrices were subjected to analyses of swelling ability and incubation studies in distilled water and simulated body fluids. The morphology and dispersion of silver nanoparticles in three dimensional structure of hydrogels was determined by means of SEM equipped with EDS analysis. Moreover, the structures of received hydrogels were evaluated applying FT-IR spectroscopy. The positive values of the cytotoxicity analysis indicate formation of innovative hydrogel matrices with enhanced antibacterial activity.
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