Phage-derived peptides improve hBMSC adhesion contact distribution and proliferation on biomimetic apatite

摘要

In addition to specifically tethering hMBSCs to mineralized biomaterials, DPI-VTK enhances cell spreading and proliferation on peptide-coated mineral substrates compared to controls. Cell spreading is greater on DPI-VTK surfaces compared to RGD-VTK in serum and greater than either RGD-VTK or BLM in the absence of serum. DPI-VTK's promotion of increased hBMSC adhesion contacts distributed at the cell periphery and increased spreading are both cell characteristics implicated in osteogenic mechano-transductive pathways. Taken together, the phage-derived peptide DPI-VTK can promote the specific recruitment of hMBSCs while encouraging favorable cell-substrate interactions and improving proliferation.