The Effect of Tumstatin Anti-tumor Peptide on Proliferation and Apoptosis of Different Cells

摘要

Tumstatin anti-tumor peptide of 19peptide caninhibit the proliferation of melanoma tumor ccll. To study itseffect on the proliferation and apoptosis of different cells andverify its no anti-tumor specificity, the base sequence of19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay, flow cytometry, chick chorioallantoic membrane (CAM),transmission electron microscopy(TEM), the tumor inhibition experiment of mice ascitic fluid transfevent H22hepatoma, the biological activity of 19peptide was studied. Experiments in vitro and in vivo identified that obtained 19peptide could inhibit proliferation of hela eell and promote its apoptosis. It also could affect proliferation of endothelial cell to some extent. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.