Design, fabrication and characterization of microphysiological systems to study drug toxicity in cardiac and adipose tissue

摘要

We designed, fabricated, and characterized microfluidic devices with various in and ex situ monitoring tools, which can be used to study drug toxicity. When fed with continuous flow our configuration and control over media perfusion enables a high density of human cardiomyocytes and adipocytes to be maintained extended time in physiologically relevant morphology. To further facilitate drug screening and assessment of direct on off-target response, multiple units of the microfluidic three-dimensional culture devices can be assembled into an array, and also combined together and with further organ on-a-chip modules, such as the liver or lung.