Effect of Centrifugal Force on Cell Culture

摘要

An effect of a mechanical force field on cell culture has been studied in vitro. C2C12 (mouse myoblast cell line originated with cross-striated muscle of C3H mouse) was cultured on a polystyrene plate coated with collagen for 13 days in the mechanical force field. To apply the mechanical force field to the cells on the plate, the plate was inserted into a centrifugal tube. The tube was placed in a conventional centrifugal machine, to set the surface of the culture plate in the parallel position to the centrifugal field. The shape and the orientation of the cells were observed with a phase contrast microscope during the cell culture. The experiments show that C2C12 extends pseudo, proliferates to the confluent manner and differentiates to myotube even under continuous stimulation of 270 times of the gravitational force.