Cana Cloning and Plant Expression Vector Construction of Soybean 11s Glycerin Gy3

摘要

The RNA was extracted from immature seeds of soybean and it is directly used in single-stranded dank synthetic reaction. According to soybean 11s glycerin Gy3 coda sequences in gene bank, a pair of primers was designed. Double-stranded coda was amplified by PCR. The amplified Gy3 coda was cloned into plasmid pMD19-T and sequenced. Fragment of the Gy3 coda in the recombi-nant plasmid was cut with Xbox Ⅰ/bah I and ligated with plasmid pbi121, and then a plant expression vector was constructed. It includes camv35 proctor, nose terminator and no-Ⅱ gene.