Design of Scaffold with Taper-Striped Pattern to Observe Durotactic Migration of Cell

摘要

The scaffold with the taper-striped pattern has been designed to observe durotactic migration of a biological cell. The surface of the scaffold with the micro taper-striped (0.05 mm < width < 0.2 mm) pattern was made by the photolithography technique. The variation of hardness of the surface of the band has been made of the materials alternately: polydimethylsiloxane (PDMS), and epoxy based negative photoresist material (SU-8). C2C12 (mouse myoblast) was seeded on the micro pattern, and incubated for 48 hours in the medium. The projected area of each cell was traced in each band at the microscopic images, and the total area was compared between bands of two kinds of materials. The experimental result shows that each cell tends to migrate to the area of the band of SU-8. The experimental system is effective to investigate durotaxis of cells in vitro.