Bioinformatic Analysis of PCSK9 Related Caspase3 Activation

摘要

To determine whether the proapoptotic effect of proprotein-convertasae cubtilisin/kexin 9 related to caspase3 activation by informatics analysis. The blank control group, the transfection regent control group, the negative control group and the 80 nmol/L PCSK9 siRNA+80μg/ml oxLDL group were established simultaneously with the participation of HUVEC-12 which was incubated with 80μg/ml oxLDL for 24hr. Expression of caspase3 was measured by western blot. In order to find the similarity of sequence and the existence of a common conservative motif, comparison was made among PCSK9, caspase8 and caspase9 by bioinformatics methods. An inhibitory effect of PCSK9 siRNA on expression of caspase3 was observed in experiments. The sequence similarity of PCSK9, caspase8 and caspase9 was 22.14%. There was a similar secondary structure such as helix, strand, turn and coil in C-terminal of PCSK9 and caspase9. The similarity also can be found in the profile of PCSK9 and caspase9, which explains the common cleft relating to their similar enzyme activity in their abdomen. Finally, there were similar motifs when comparing PCSK9 with caspase9, while the proapoptotic capacity of PCSK9 has association with its activation effect on caspase3.