Identification of Picking Time Of Mulberry Leave by UV Two-Dimensional Correlation Analysis Based On pH Perturbation

摘要

The picking time of mulberry leave has an important influence on its efficacy. The UV-Vis two-dimensional correlation spectroscopy based on pH perturbation was designed to identify the picking time of mulberry leave. Water extractiings from mulberry leave were mixed with Britton-Robison buffer (pH 2,3,4,5,6,7,8 9,10and 11), and its UV-visible spectra was recorded. The raw spectra were preprocessed with 15 points first-order differential, and then were analyzed by two-dimensional correlation. The synchronization maps of four picking time were similar;the asynchronous maps were different in peak number and location from which identification purpose were achieved. It was a novel method, two-dimensional correlation analysis based on pH perturbation, of identifying picking time of mulberry leave.