Effect of GRGDSP-Alginate in Alginate Foam for 3D Cell Culture Compared to Cells Affinity to FITC-GRGDSP

摘要

For some cell types was the cell proliferation rate effectively promoted by addition of GRGDSP covalently bound to alginate. The differences seen in binding capacity of FITC-GRGDSP may reflect the dependency of attachment peptides for cell proliferation in an inert alginate matrix and the presence of receptors available for binding on the cell surfaces. Evaluating the cells' affinities for different peptide sequences and then covalently couple the relevant sequences onto alginate may be of high value, as this knowledge may be used to optimize the cell culture system and control cell interaction with a synthetic ECM such as the alginate based NovaMatrix-3D .