NovaMatrix-3D~(TM)- 3D Cell Culture and Formation of Multi-Cellular Structures in Alginate Foams

摘要

Use of NovaMatrix-3D™ with concomitant in situ immobilization of cells results in a 3D model with the potential to approximate cell growth and architecture within tissues or tumors. Cells are uniformly entrapped into the foam matrix by a gentle and fast technique. Cell visualization can easily be performed due to matrix transparency. Cells seem to freely form multi-cellular structures within the matrix and intact structures may easily be isolated under physiologic conditions. The immobilized cells can be treated with drugs or other agents in cell survival or cytotoxicity experiments since the alginate matrix is permeable for small and medium sized molecules. The formed structures may also be harvested for further investigation.