Efficient DNA transfection is crucial for DNA vaccine delivery, as low efficacy is often observed in manyin vitro and in vivo studies. To overcome the problem due to cell barriers, the gene vector can be associatedwith narrow size superparamagnetic nanoparticles, followed by application of an external magnetic field totarget the vector to its desired direction. This technique has been proven to result in excellent celltransfection level and to speed up the duration of the process from hours to a few minutes and minimizethe vector dose. Thus, the undesired toxicity of magnetic nanoparticles is also reduced. The objective ofthis study is to improve malaria DNA vaccine delivery with magnetofection by using the membraneassociated 19-kDa COOH terminal fragment of merozoite surface protein (MSP119) that plays a criticalrole in plasmodium immunity and is now a leading malaria vaccine candidate.
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