An Analysis of Synaptic Transmission and Its Plasticity by Glutamate Receptor Channel Kinetics Models and 2-Photon Laser Photolysis

摘要

We predicted the rate constants of AMPA receptor channels kinetics models of neural cells which could induce the change in efficacy of synaptic transmission by computer simulation. Excitatory postsynaptic currents (EPSCs) were reconstructed by computer calculation with the proposed kinetics models. Moreover, electrical responses were measured from the cells by using 2-photon laser uncaging. It was shown that the properties of the evoked current responses by photolysis and those obtained from spontaneous synaptic currents have the similar properties which indicates that the present AMPA receptor channel models mediate the evoked responses by laser photolysis We investigated and proposed the possible rate constants, which could explain the changes in the EPSCs amplitude during LTP/LTD without changing the waveform, and corresponding physiological elements to them. Moreover, it is suggested that the present method based on kinetics models could be used for the investigation of other experiments evoked by laser photolysis.