Analysis of a Ribosomal RNA Operon (rrn) from 'Candiatus Liberibacter asiaticus'

摘要

A 5 005 bp rrn DNA sequence of "Candidatus Liberibacter asiaticus" was obtained by PCR using primers conserved to Rhizobiaceae of alphaproteobacteria. The rrn locus consisted of a 16S rRNA gene (rrs) , an intergenic transcribed spacer (ITS) containing two genes of tRNAlle and tRNA Ala, a 23S rRNA gene (rrl) , an ITS without tRNA gene and a 5S rRNA gene (rrf). like rrs, rrl and rrf were also related (89% -90%)to those of the members of Rhizobiaceae. Interestingly, the genes of tRNAlle and tRNAAle were more related to those of nonRhizobiaceae alpha-proteobacteria. The non-tRNA gene regions of the 16S-23S ITS and 23S-5S rRNA ITS did not share significant similarity to any known nonLiberibacter DNA sequences, and could be used for specific and efficient detection of ' Ca. L. asiaticus'.