Immobilized Metal Chelate Affinity Hollow Fiber Membrane for Lysozyme Adsorption

摘要

PP hollow fiber membrane was firstly modified by oxygen plasma at low temperature and the polar groups such as ester, aldehyde, ketone, carboxylic acid, detected by IR and XPS, had been attached to the membrane after plasma treatment. Then, the modified PP membrane was activated by epichlorohydrin, couple dwith IDA (iminodiacetic acid) and chelated with copper ion. The XPS analysis showed that the N_ls peak was on the coupled PP membrane. The batch isothermal adsorption of lysozyme on immobilized metal chelate affinity (IMA) membrane was based on Langmuir isotherm model. Furthermore, a porous PP hollow fiber affinity membrane module was prepared for dynamic lysozyme adsorption.The breakthrough curve of lysozyme at three different feed concentrations showed that the saturation time decreased with the increasing of feed concentration.