Fluorescence dynamics of upper layer of human skin under UV laser irradiation

摘要

Abstract: In present work the temporal behavior ofautofluorescence of upper (30 - 40 $mu@m) layer ofhuman skin under continuous 337 nm laser exposure wasinvestigated. The samples were prepared using epidermisstripping technique. Kinetic curves of autofluorescencedecay at 420 nm, 440 nm, and 460 nm have been obtained.In double exponential approximation the parameters ofautofluorescence decay have been evaluated. Under theassumption that different terms in double exponentialapproximation correspond to different individualfluorophores the contribution of fast bleaching, slowbleaching, and non-bleaching components to fluorescencespectrum of the sample at 420 nm, 440 nm, and 460 nmhave been calculated.!5