Effect of Vortex Flow on Cultured Cells in Vitro

摘要

An effect of flow on cell culture has been studied in vitro. A silicone disk was placed on the bottom of a culture dish to make a doughnut-shaped canal. The dish was placed on a tilted plate, which rotates to make a vortex flow around the silicone disk with swing motion. Variations were made on the dimension of the canal of the silicone disk. Three kinds of cells were cultured in the vortex flow of Dulbecco's Modified Eagle's Medium for five days: C2C12 (mouse myoblast), L6 (rat skeletal muscle cell), and normal chondrocyte (Takara-bio). The experimental results show that the cells adhere in the low shear area in the first stage, and that cells line to the perpendicular direction to the flow in the second stage. C2C12 is more adhesive than chondrocyte on the culture dish with collagen coating in the flow.