The aim of this paper was to explore the effect of extrusion and enzyme co-extrusion on the structure of pea protein. Pea protein was pretreated with extrusion and enzyme co-extrusion. The effect of pretreatment of extrusion and enzyme co-extrusion on pea protein was further studied by measuring the microstructure, disulfide bonds content, protein secondary structure, functional groups and amino acid composition of the optimal substrate. The results showed that the extrusion and enzyme co-extrusion could cause significant change to the microstructure of pea protein, reduce the content of disulfide bonds, cause change to the content of secondary structure and the composition of amino acids, but no change to the functional groups.
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