PROTEIN ENRICHMENT AND CELLULASE PRODUCTION OF PANGOLAGRASS BY SOLID STATE FERMENTATION

摘要

The annual production of pangolagrass, Digitaria decumbens Stent, was 2.41–3.86×10 5 ton during1990 to 2001 in Taiwan. To improve the quality of pangolagrass for animal feeding, cellulolyticmicrobes were isolated from various sources and cultivated by solid state fermentation. The optimalconditions for protein enrichment and cellulase production were recorded an initial moisture65-70%, initial pH 6.0-8.0, supplementation with ammonium sulfate and minerals, and cultivationat 30 o C. The protein content in non-sterilized substrate enriched from 6.48% to 8.46, 8.52, 9.68,9.52, 11.99 and 12.25% with the inoculation of Mucor hiemalis 2216, Aspergillus niger, isolateSC99, Streptomyces thermonitrificans NTU-88, isolate SC2, and Myceliophthora lutea CL3 for 29days incubation, respectively; while the protein contents in sterilized substrate were 8.26, 9.82, 9.68,11.20, 16.85 and 18.89%, respectively. The in vitro digestion improved from 4.11-4.38% to7.53-17.82%. Each gram of dry substrate yielded 1.02-3.76 U Avicelase, 1.50-4.98 U CMCase and1.50-5.18 U β -glucosidase. M. Lutea CL3 was the best strain in the improvement of the quality ofpangolagrass for animal feeding by solid state fermentation.