The Photosensitizer Talaporfinum Caused Microvascular Embolization for Photodynamic Therapy

摘要

Photodynamic therapy (PDT) has been evolving rapidly in the recent years. A second-generation Photosensitizer mono-1-aspartyl chlorine 6 (Talaporfin/Npe6/ME2906, Japan Meiji Seika, Ltd.) has been sanctified for the lung cancer clinical PDT by the Japan Ministry of Health, Labor and Welfare. In this paper, Talaporfm was injected to the implant cancer of a mouse a Talaporfm dose of 5mg/kg through intravenous. After 6 hours, the fluorescence images of the mouse were observed with a microscope and a 664 nm diode laser. Effects of therapy were clarified using the different irradiation energies of the laser (50, 100, 200 J/cm~2). Both in plasma and in cancer, the concentrations of Talaporfm were analyzed using High Performance Liquid Chromatography (HPLC). Authors find that the higher concentrations of Talaporfm in plasma, the better PDD effect. It is experimentally verified that local microvascular embolisms in the cancer are formed for photodynamic therapy after the Talaporfin injection and the laser irradiation.