Determination of Thymosin β_4 and Protein in Human Wound Fluid after Abdominal Surgery

摘要

Wound fluids were collected up to 60 h after abdominal surgery. Immediately after obtaining the wound fluid by Robinson drainage, wound fluid was centrifuged to remove blood cells and inflammatory cells. The concentration of total protein as well as of thymosin β_4 was determined in the cell-free supernatant solution. Total protein concentration decreased from about 50 g/L to 30 g/L within 60 h after surgery. After surgery we observed a concentration of up to 20 mg thymosin β_4 per liter decreasing to about 1 mg/L with time. Neither thymosin β_(10) nor oxidized thymosin β_4 was detected in human wound fluid.