Protein and antigen microarray preparation by using agarose-gel substrate and characterization

摘要

To investigate a novel solid support adapted to different kinds of biomolecules cross linking, and evaluate the experimental conditions of protein and antigen micoarray preparation. 3 different Agarose-gel film on the surface of APTES-modified slides were generated (surface-1, surface-2 and surface-3, respectively. Results presented that reactive aldehyde groups were successfully induced onto the gel-substrate, and surface-3 had an optimal performance of protein immobilization and measurements. There had no significant difference among different temperatures (i. e. 37℃, 25℃ and 4℃, respectively) influenced on this gel-microarray in employment. Characterization results provided direct evidences that proteins immobilized on gel surface could be clearly observed, and Nls peak could be also detected after proteins attached. This investigation illustrated the mechanisms of which biomolecules could be firmly immobilized on gel-film. It was important that surface derived active aldehyde groups and 3-dimensional structure were the main reasons for the improvement of biomolecules immobilization.