IMMUNOSENSOR FOR HERBICIDE 2,4-DICHLOROPHENOXYACETIC ACID

摘要

The improved highly sensitive piezoelectric irnmunosensor has been developed and evaluated using a model interaction of antibody with the model hapten - herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of 4-aminothiophenol were formed on smooth and rough crystals coated with gold or silver electrodes. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. The competitive assay of the herbicide 2,4-D achieved the limit of detection of 2 ng/L using the monoclonal anti-2,4-D antibody F6/C10.