PRODUCTION OF MUTANTS USING THE RAPESEED DOUBLED HAPLOID SYSTEM

摘要

Over the past 20 years, researchers have made major progress in haploid technology and protocol development for the oilseed Brassicas. Availability of refined protocols for the production and manipulation of haploid tissue has attracted the attention of breeders, geneticists and biotechnologists alike. Efficient and rapid production of haploid embryos from immature pollen grains provides an opportunity to combine in vitro mutagenesis with selection for novel genotypes from large populations of genetically unique haploid and doubled haploid plants. Chromosome doubling in haploids produces fertile diploid true breeding plants homozygous for all traits. Application of chemical or physical mutagens to microspore cultures provides genetic alterations for selection. Microspore technology has been utilized in the production of rapeseed lines resistant to numerous herbicides and has modified the fatty acid contents of seed oil and improved the quality of seed meal.