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Optimization of doubled haploid production in burley tobacco (Nicotiana tabacum L.).

机译:白肋烟(Nicotiana tabacum L.)中单倍体产量翻倍的优化。

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摘要

Doubled haploidy (DH) is a plant breeding technique that is often utilized by plant breeders to minimize the time required to reach homozygosity in breeding lines. The first objective of this study was to compare two methods of generating DH lines in tobacco (Nicotiana tabacum L.). Inbred burley tobacco varieties TN 90LC and GR 149LC were used to produce both androgenic derived doubled haploids (ADDH) and maternally derived doubled haploids (MDDH). The relative agronomic performance of TN 90LC and GR 149 LC ADDH and MDDH lines was compared when used either as pure-line cultivars or when used for the production of the KT 204LC and TN 97LC hybrid cultivars, respectively. The ADDH method was more efficient than the MDDH method in generating large numbers of haploid plants. On average the ADDH TN 90LC population was statistically inferior to the inbred TN 90LC for several agronomic traits; this inferiority of the ADDH method was not observed in the GR 149LC populations. For both genotypes, the MDDH populations were comparable to the inbred parental genotypes. The ADDH method was inferior for TN 90LC, but several individual TN 90LC ADDH lines were equal or superior to the inbred source. The agronomic variability observed in both ADDH and MDDH lines was decreased when they were used to produce hybrid cultivars. Less variation was observed in the DH-derived hybrids KT 204LC and TN 97LC compared to the ADDH and MDDH TN90LC and GR149LC parental lines, respectively. The significant inferiority of ADDH TN 90 lines in comparison to inbred TN 90LC was not observed in the ADDH derived KT 204 population compared to KT 204LC. The second objective of this study was to compare DH Lines derived from an F1 breeding population versus DH lines derived from a segregating F2 population where plants used for DH were pre-screened for quantitatively inherited resistance to soil-borne diseases black shank (Phytophthora nicotianae) and/or Fusarium wilt (Fusarium oxysporum f. nicotianae). There was a clear difference in susceptibility to black shank between the F1 and F2 derived DH populations, both in terms of average disease incidence, and more importantly, in the percentage of individual lines displaying high disease resistance. For two different burley crosses, DH lines derived from the F1 generation were considerably more susceptible to black shank than DH lines derived from the F2 generation. No differences in the incidence of Fusarium wilt were observed between DH lines of F1 and F2 generations; this was likely due to low overall disease incidence. Although delaying the DH process in tobacco from the F1 to the F2 generation could add time to the development of homozygous breeding lines, the delay may be offset by having to screen fewer finished DH lines to identify superior lines.;Keywords: Tobacco breeding, doubled haploidy, black shank, Fusarium wilt.
机译:双倍体单倍体(DH)是一种植物育种技术,植物育种人员经常利用它来最小化在育种系中达到纯合性所需的时间。这项研究的第一个目标是比较在烟草(Nicotiana tabacum L.)中产生DH系的两种方法。自交白肋烟品种TN 90LC和GR 149LC用于生产雄激素衍生的双单倍体(ADDH)和母体衍生的双单倍体(MDDH)。当分别用作纯系品种或用于生产KT 204LC和TN 97LC杂交品种时,比较了TN 90LC和GR 149 LC ADDH和MDDH品系的相对农艺性能。在生成大量单倍体植物方面,ADDH方法比MDDH方法更有效。平均而言,ADDH TN 90LC的几个农艺性状在统计学上低于近交TN 90LC。在GR 149LC人群中未观察到ADDH方法的劣势。对于这两种基因型,MDDH群体与自交亲本基因型相当。 TN 90LC的ADDH方法不如TN 90LC,但几个单独的TN 90LC ADDH系与近交源相同或更高。当将它们用于生产杂交品种时,在ADDH和MDDH品系中均观察到了农艺变异性的降低。与ADDH和MDDH TN90LC和GR149LC亲本相比,在DH衍生的杂种KT 204LC和TN 97LC中观察到的变异较小。与自交系TN 90LC相比,ADDH TN 90系与自交系TN 90LC相比没有显着劣势。这项研究的第二个目标是比较来自F1育种种群的DH品系与来自分离F2种群的DH品系,其中预先筛选了用于DH的植物对土传疾病黑胫病(Phytophthora nicotianae)的定量遗传抗性和/或枯萎枯萎病(Fusarium oxysporum f。nicotianae)。从平均发病率,更重要的是,在显示出高抗病性的单个品系中,F1和F2衍生的DH群体对黑胫病的敏感性存在明显差异。对于两个不同的白肋烟杂交,来自F1代的DH系比来自F2代的DH系更容易受到黑胫病的影响。 F1代和F2代DH系之间枯萎病的发生率没有差异。这很可能是由于总体疾病发病率较低。尽管将烟草的DH过程从F1推迟到F2可能会增加纯合育种系的开发时间,但可以通过筛选较少的最终DH系以鉴定优良系来弥补这一延迟。单倍体,黑柄,镰刀菌枯萎。

著录项

  • 作者

    De Oliveira, Ezequiel.;

  • 作者单位

    University of Kentucky.;

  • 授予单位 University of Kentucky.;
  • 学科 Plant sciences.;Agronomy.
  • 学位 Ph.D.
  • 年度 2016
  • 页码 180 p.
  • 总页数 180
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

  • 入库时间 2022-08-17 11:43:26

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