Wide field speckle imaging for the investigation of cerebral blood flow in vivo in murine model of obesity

摘要

The morphological and functional changes in cerebral blood vessels network is not well characterized in mice models ofobesity. In order to study the hemodynamics of these models at rest and during sensory stimulation, we have developed amulti exposure speckle imaging system. It allows wide field superficial imaging of blood flow of the mice cortex. Wehave characterized the performances of the system using microfluidic phantoms. The capacity of the technique toretrieve accurate relative flow values was studied as a function of the diameter of vessels and the scatterersconcentration. New biological data have been obtained in mice models of obesity (high fat diet mice) at rest and undersensory activation.