SEPARATION OF AMINO ACID BY SIMULATED MOVING BED USING COMPETITIVE LANGMUIR ISOTHERM

摘要

The separation of two amino acids, phenylalanine and tryptophan, was carried out by laboratory simulated moving bed (SMB) chromatography. The SMB process consists of four zones and each zone has 2 columns. Triangle theory was used to obtain operating conditions for SMB. Mass transfer coefficient was obtained by the best-fit values of two amino acids comparing simulation with experimental pulse data. The competitive adsorption isotherms of two amino acids were obtained by single and binary frontal analysis considering competition between two components. Competitive Langmuir isotherm from single-component frontal chromatography was used in the first run. In the second run the isotherm from binary frontal chromatography was used and the flow rate of zone 1 was modified to improve purity. Compared to the first and second run, competitive Langmuir isotherm from binary frontal chromatography shows good agreement with the experimental results. Also, adjusting flow rate in zone I increased the purity of products. The purity of phenylalanine at raffinate was 99.18% and that of tryptophan at extract was 99.67%.