Paraffin versus frozen sectioning in the histologic evaluation of biomaterial implants - a pilot study

摘要

Paraffin sections are not inferior to frozen sections to histologically evaluate material implants. If it is regardless whether paraffin or frozen sections are taken regarding the possibility of histological evaluation, clearly paraffin sections should be performed. The reason is the enlarged methodical possibility by e.g. producing histological stains which play a major role to help evaluate various tissue reactions like inflammation. A further important aspect is the far more easy way of storage of material for paraffin sections compared to frozen sections. To store frozen material is expansive, sophisticated and bears the risk of material loss if e.g. the fridge breaks or power failure occurs. Tissue treated with paraffin, by contrast, is easy and cheap to store and sections are easy to reproduce since the slice is always cut from the same surface. Altogether, paraffin sections are as good as frozen sections and should then be the preferred method since tissue is easier to store and the diagnostic spectrum is broadened e. g. by the possibility of immunohistological staining.