In vitro degradability, bioactivity and cell responses to mesoporous magnesium silicate for bone regeneration

摘要

Mesoporous magnesium silicate (m-MS) was firstly synthesized, and the in vitro degradability, bioactivity and primary cell responses to the m-MS were investigated. The results suggested that the m-MS with mesoporous channels of around 5 nm possessed the high specific surface area of 451.0 m2/g and pore volume of 0.41cm3/g as compared with magnesium silicate (MS) without mesopores of 75 m2/g and 0.21 cm3/g, respectively. The m-MS could absorb a large number of water with the water absorption of 74 % as compared with MS of 26 %. In addition, the m-MS could degrade in Tris-HCl solution with the weight loss ratio of 40 w% after immersion for 70 days. The m-MS had good in vitro bioactivity, which could induce apatite formation on its surfaces when soaked into simulated body fluid (SBF), and the rate of apatite formation for m-MS was obviously faster than MS. The cell experiment results revealed that m-MS could obviously promote proliferation and differentiation of the MC3T3-E1 cells on its surfaces than MS with time, and exhibited normal cell morphology, indicating excellent cytocompatibiliry. This study suggested that the mesoporous magnesium silicate with high specific surface area and pore volume had suitable degradability, good bioactivity and biocompatibility, which might be an excellent candidate of a biomaterial for bone regeneration.