Preparation, bioactivity, degradability and primary cell responses to an ordered mesoporous magnesium-calcium silicate

摘要

An ordered mesoporous calcium-magnesium silicate (om-CMS) for bone repair was synthesized and characterized, and the in vitro bioactivity, degradability and primary cell responses to the om-CMS were investigated. The results showed that the om-CMS possessed a large surface area of 1017 m~2/g and highly ordered hexagonal channel pores of around 7 nm. The om-CMS had good bioactivity, which could induce apatite formation on its surfaces after 5 d of soaking in simulated body fluid (SBF), and the om-CMS exhibited enhanced bioactive behavior, with even faster apatite formation than calcium-magnesium silicate (CMS). Furthermore, the om-CMS could be degradable in Tris-HCl solution with the weight loss ratio of 40wt.% after immersion for 84 d. In cell cultural experiments, the results revealed that om-CMS could promote proliferation and differentiation of the MC3T3-E1 cells with time, and the cells with a normal morphology spread well on the om-CMS surface, which showed no negative effects of the substrate on cells. In conclusion, the results demonstrated that the degradable om-CMS with good bioactivity had the ability to support cell attachment, proliferation and differentiation, and exhibited good cytocompatibility.