Bioactive Compounds from Okra Seeds: Potential Inhibitors of Advanced Glycation End Products

摘要

Advanced Glycation End Products (AGEs) have been associated with the micro-vascular complications in diabetes and other age-related neurodegenerative diseases. We have investigated the effect of glycosylation of bovine serum albumin (BSA) in the presence of okra seed extracts. The degree of protein glycation with glucose was assessed by tryptophan AGE, AGE-induced cross-linking by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and NanoDrop spectrophotometry. Fluorescence spectra (excitation at 360 nm and read at 460nm) of BSA solution incubated for 90 days with okra seed extracts showed significant inhibitory potential (45-50%) at 0.1mg/ml concentration in a dose dependent manner. Intensity of fluorescence spectra combined with densitometry measurements exhibited 50% inhibition of glycation of BSA. We propose that the fluorescence emission spectra were altered by glycation when incubated with okra seed extracts and thus inhibited the advanced glycation end products. Further studies are needed to understand the bioactive compounds present in okra seed extracts in in vivo models.