Digital Micromirror Device-based High-speed and High-resolution Quantitative Phase Imaging

摘要

We demonstrate a high-speed and high-resolution quantitative phase imaging method by implementing a syntheticaperture technique through using digital micromirror devices (DMDs). DMDs provide high speed steering of theillumination beam angle upon the sample that induces sample frequency shift, which is the basis of achieving highresolution in the quantitative phase imaging (QPI) system. With a high-speed camera for image acquisition, our QPIsystem achieves synthetic aperture imaging at >25 frame per second (fps). The high-speed imaging capability of thesystem allows for better observation of samples dynamics, especially in live biological structures, where motions couldresult in degraded imaging quality. In experiments, our synthetic aperture-based QPI system has resolved sub-diffractionlimited structures of 220 nm periods and quantified red blood cell membrane fluctuations, which opens new avenues inmaterial metrology and biological imaging applications.