High Resolution 3D-Reconstruction from Serial Sections. Microscope Instrumentation, Software Design and its Implementations

摘要

This paper describes a set-up for high-resolution imaging with a conventional microscope that allows producing a 3D-image set from which a 3D model can be derived. The 3D-image set, is de facto, a gray value voxel model. In order to obtain such 3D image set a good administration needs to be maintained at acquisition and moreover, the acquisition must be realized in two steps. High-resolution images are built of image tiles and sophisticated algorithms are required to build a coherent image from the tiles.