The primary application of AF/PSTM in the imaging of biologic sample

摘要

Our research group has recently developed a new type scanning probe microscope - AF/PSTM. Using this setup, the optical false image caused by the inclination of sample surface can be eliminated; the optical image and the topography image are separated; and also two optical images (refractive index image, transmittivity image) and two AFM images (topography image, phase image) are obtained during one scanning. As a primary biologic application, this setup is engaged in the imaging of some biologic samples. The primary images of these biologic samples are obtained. Due to the advantage of AF/PSTM, four images can be acquired at the same time during one scanning. Consequently more information of sample is given by comparing these images. This work shows that the AF/PSTM may be improved to be a useful tool in biology research.