Magnetic Field Effects on Crystallization of Thaumatin by LLIP Method Ⅱ

摘要

Many crystallization techniques have been studied to produce a huge protein crystal with high quality for XRD analysis in the field of drug development. Liquid-liquid interfacial precipitation (LLIP) method is one of new crystallization techniques and it is available to control the crystal size and polymorph[l]. When two kinds of solvent are stacked to make their liquid-liquid interface, the 2-dimensional interface gets supersaturated due to a mutual diffusion. Then a crystal is formed and precipitated at around the interface. Alarge number of studies using LLIP method have been reported to make a protein crystal in space. Meanwhile a vertical high-gradient magnetic field is able to provide a reduced or hyper-gravity environment. In this paper, thaumatin protein was crystalized by LLIP method under the influence of vertical magnetic field with gradient, and its magnetic field effects on size of crystal was studied.