Design, production, and isolation of the Family 44 glycoside hydrolase, Clostridium acetobutylicum endoglucanase

摘要

The specific activity observed in the concentrated broth in the ammonium sulfate saturation range of 35-60% was lower than that for less than 35% and this is concerning but based on the Congo Red assay, SDS-PAGE results, and specific activities of the dialyzed cell free broth, the weight of data shows that the target protein precipitates in the range of 40-60%. The apparent drop in specific activity with the ammonium sulfate precipitation needs to be investigated further to identify it is occurring due to loss of protein, denaturation, loss of activity due to experimental conditions or errant data.