Rapid Determination of Protein, Oil, Moisture, and Isoflavones Contents of Single Soybean Seeds by Fourier Transform Near Infrared Reflectance Spectroscopy

摘要

Composition analyses on both intact single seed and single half seed soybean samples can be successfully performed with state-of-the-art FT-NIR instruments. Spectral variations and light scattering effects were effectively corrected by pre -processing procedures including baseline correction and light scattering correction. Our calibrations are characterized with high correlation coefficients and low prediction errors, with R>99% and SEP<1% for all four components. Seed-to-seed variations in protein, oil, moisture, and isoflavones content can thus be monitored for the first time on individual soybean seeds of selected soybean accessions.