Differential two-color x-ray radiobiology of membrane/cytoplasm in yeast cells and lymphocytes

摘要

Abstract: An overall approach into the differential investigationof membrane/cytoplasm related metabolism and ofcell-cycle of yeast cells after two color soft X-rayirradiation is presented; the soft X-rays beinggenerated in trains of picosecond pulses bylaser-plasma interaction. The two color X-raydifferential technique is based on the generation ofapproximately 0.6 KeV X-rays which are deposited onlyin the membrane-wall complex switching off theanaerobic (fermentative) activity of yeast cells and onthe generation of approximately 1.2 KeV X-rays whichare mainly deposited in the cytoplasm, mitochondria andnucleus of yeast cells, mainly affecting the aerobicmetabolism. A synergetic analysis of the metabolism isdiscussed, mainly founded on the recording of differentcorrelated metabolic parameters, both on-line anddelayed. Among the relevant access, pressure monitoringin batch samples acquire a dominant role allowing theidentification of metabolic oscillation, that representa marker of physical and chemical actions performed onthe samples at different times. The experience acquiredon yeast cells metabolism is being used to investigatelymphocytes metabolism and the related oscillatoryproperties of relevant enzymatic complexes. Actuallyeven if it is not exactly the same as the mammaliansituation, it should really propel the whole fieldforward. !12