Detecting HIV-1 Mediated Neuronal Dendritic and Synaptic Damage by a MCLP Data Mining Method

摘要

The ability to identify neuronal damage in the dendritic arbor during HIV-1 associated dementia (HAD) is crucial for designing specific therapies for the treatment of HAD. To study this process, we utilized a computer based image analysis method to quantitatively assess HIV-1 viral protein gpl20 and glutamate mediated individual neuronal damage in cultured cortical neurons. Changes in the number of neurites, arbors, branch nodes, cell body area, and average arbor lengths were determined. We proposed a two-class model of multiple criteria linear programming (MCLP) to classify such HIV-1 mediated neuronal dendritic and synaptic damages. Given certain classes, including treatments with brain derived neurotrophic factor (BDNF), glutamate, gpl20 or non-treatment controls from our in vitro experimental systems, we used the two-class MCLP model to determine the data patterns between classes in order to gain insight about neuronal dendritic damages under different treatments. This knowledge can be applied in principle to the design and study of specific therapies for the prevention or reversal of neuronal damage associated with HAD.