A qPCR-based Analysis of Environmental Water Samples for E. coli and toxigenic E. coli

摘要

A risk of waterborne disease outbreaks still exists in developed countries, and Wisconsin is nornexception. Recent research has indicated that the standard culture-based methods used today torndetermine the risk of waterborne disease in drinking and recreational waters are less than perfectrnfor protecting public health. Utilizing molecular methods, specifically quantitative polymerasernchain reaction (qPCR), may be one approach to improving the protection of public health byrnreducing analysis times. Therefore, the objective of this research project was to optimize qPCRrnassays for generic E. coli and toxigenic E. coli in recreational and drinking waters that willrnprovide rapid, unbiased results.