Organ-explanted bionic simulator (OBiS): Concurrent microcardiovascular anastomosis of chick embryo

摘要

In this paper, we newly propose an organ-explanted bionic simulator (OBiS) using an isolated organ tissue. We use a heart isolated from the chick embryo for proposed simulator. To achieve the OBiS, we perform the concurrent microvascular anastomosis by using suction-induced vascular fixation (SVF) method for easily connection to artificial tubes with blood vessels led to the explanted chick's heart. Through the experiment with a simulated cardiovascular, it is confirmed that vessels can be aligned by using the proposed method. In addition, we have succeeded in connecting the Alg tubes with the blood vessels led to the heart of chick embryo. OBiS is available for the evaluation of new drugs immune response not only without using large animals but also as a response of organs beyond cellular level.