Effects of Fe3O4 nanoparticles on the transcription of genes with iron-responsive elements

摘要

In this paper, we checked the transcription of 24 murine genes containing iron-responsive elements in Fe3O4 magnetic nanoparticles treated mouse macrophage RAW264.7 cells using cDNA microarrays and real time-PCR. The results showed that only 7 genes differentially expressed (fold change < −1.5 or > 1.5) after incubated with 100 µg/ml Fe3O4 magnetic nanoparticles. Gene Ontology analysis of these genes containing iron-responsive elements demonstrated that the differentially expressed genes, including Tfrc, Slc11a2 and Slc40a1, played a crucial role in iron ion homeostasis. And the non-differentially expressed genes (−1.5< fold change <1.5) were related to cell biosynthesis and metabolism, including acetyl-CoA metabolic process, peptide metabolic process, and macromolecule catabolic process.